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Why is it important to not dilute an initial sample before it has been loaded onto the chromatography column?

Question:

Why is it important not to dilute the initial sample before it has been loaded onto the chromatography column?

Chromatography

Chromatography is a process of separation of the mixture and the process of chromatography includes the mobile phase and the stationary phase. Chromatography is one of the major separation techniques in chemistry.

Answer and Explanation:

It is important not to dilute the initial sample before it has been located onto a chromatography column so that the matrix effect could be reduced and saturation of the detector should be avoided. It is also done to make sure that saturation remains in the range of the calibration curve.

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